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New reliable methods to identify sterile Q-flies

We would like to recommend the reading of the paper that Dr Bishwo Mainali and coauthors recently published in the Journal of Pest Science.


Read the publication


"Stable isotopes for reliable identification of wild and mass-reared Queensland fruit flies in sterile insect technique programs"


Bishwo Mainali, Anita S. Andrew, Phil Taylor & Polychronis Rempoulakis

Abstract


Queensland fruit fly is one of the most economically important horticultural pests in Australia. Sterile insect technique (SIT) is now being reconsidered and upscaled to combat this pest so reliable discrimination of released sterile Q-flies from wild flies in monitoring traps is important for effective SIT operations. Stable isotopes provide a permanent chemical marker to discriminate sterile and wild flies when dye marking is unclear. In this study, we compared the isotopic ratios of carbon and nitrogen between Q-flies reared on different larval diets and wild flies collected from diverse locations in Australia and New Caledonia. Finally, we conducted a release–recapture study to corroborate differences in stable isotope C and N ratios in laboratory-reared and wild Q-flies. The δ15N values obtained from wild and laboratory Q-flies showed high variability that is likely related to the food source of the larval and/or adult stage and do not offer an effective means to discriminate between sterile and wild Q-flies. The δ13C values of examined wild Q-flies ranged from − 27.46 to − 24.37‰ VPDB, whereas those from laboratory-reared, released and recaptured Q-flies ranged from − 25.73 to − 19.26‰ VPDB. Differences in δ13C values resulted in 100% correct classification of wild flies and 96.88% correct classification of released flies. Measurements of intrinsic δ13C values offer a precise tool to discriminate between sterile and wild Q-flies in SIT programs, regardless of the composition of the larval or adult pre-release diets.



Photo: Dr Bishwo Mainali (Source: Chris O'Connor).

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